Development of High-Throughput DNA Sequencing Techniques to Improve and Advance Environmental Monitoring and Bioassessment
Currently, biological monitoring programs for rivers, streams and other aquatic areas focus primarily on identifying aquatic macroinvertebrates such as insects, snails and worms. These efforts can take 6 to 12 months. However, because these aquatic organisms leave molecular-level DNA 'footprints' in the environments where they live, scientists can use molecular methods, such as DNA barcoding, to obtain reliable information about the species in a given area. DNA barcoding uses short genetic markers in an organism's DNA to identify it as belonging to a particular species. It is also be possible to use these DNA-based techniques to accurately identify much smaller aquatic organisms, such as algae and bacteria.
EPA scientists are now using new molecular technology, such as high-throughput DNA sequencing to monitor and assess aquatic ecosystems. Sequencing technology is used to determine the exact order of nucleotides in a DNA sample. Nucleotides are the building blocks of DNA. With this cutting-edge technology, scientists can process multiple water samples simultaneously and create a comprehensive list of organisms present at that site. In fact, samples from up to approximately 400 sites could be analyzed simultaneously, which would drastically reduce laboratory testing costs.
EPA scientists are developing a method to provide robust, reliable and timely bioassessments for samples from streams and marine coastal sites. Using DNA sequencing technology, they are working on ways to identify large invertebrate species and comparing their results with the findings of taxonomists who have identified the species individually. For the coastal samples, they are looking at soil samples to see if they can identify large and small invertebrate species using DNA barcoding techniques.
Because these methods will provide more data than standard bioassessment protocols, EPA scientists think these new techniques will not only help determine the condition of specific aquatic ecosystems, but might also detect whether a site is improving or degrading. These methods might also help scientists detect the presence of pollutants in streams.
In Figures 1 and 2 below, the likely benefits of using genomic community data are blue. The darkly-shaded boxes indicate the areas where current efforts are focused.
Publications
- Does DNA barcoding improve performance of traditional stream bioassessment metrics?
- Cryptic biodiversity in streams: a comparison of macroinvertebrate communities based on morphological and DNA barcode identifications
- Incorporation of DNA barcoding into a large-scale biomonitoring program: opportunities and pitfalls
- Can DNA barcodes of stream macroinvertebrates improve descriptions of community structure and water quality?
- Environmental Barcoding: A Next-Generation Sequencing Approach for Biomonitoring Applications Using River Benthos