PIPs: Brain on a Chip

EPA scientists have long been interested in the potential effects of chemicals on developing brains in embryos, infants, and children. However, traditional methods for testing these effects are expensive, time-consuming, and rely on animal models. Because of that, many chemicals have not been studied using these methods, resulting in gaps in what we know about chemicals found in the environment.

In 2013, Dr. Timothy Shafer’s team at EPA received PIP funding to develop a new, lower cost approach to quickly detect when chemicals may cause developmental neurological changes. The team grew a network of brain cells in a laboratory and measured the network’s electrical activity using an array of tiny electrodes. This lab-grown network simulates the electrical activity of a human brain; chemicals that cause changes in this “brain on a chip” are flagged as potential candidates that could impact developing brains in humans.

With the help of a jump start from PIPs, the “brain on a chip” has become an integral part of EPA’s Chemical Safety for Sustainability research program.

“PIPs allowed us to really get this technology up and running,” Dr. Shafer said. “It allowed us to generate enough data to support further development and use of this approach.”

Today, EPA is using the method to test new and existing chemicals (including high priority contaminants such as PFAS), and the data are informing EPA’s regulatory decisions. For example, according to Dr. Shafer, “brain on a chip” informed a decision about a new pesticide and saved about a year and a half of EPA staff time and over $1 million compared to traditional methods.

Read about more PIPs success stories and get all the latest news from PIPs on our PIPs homepage.