LAND AND SEA RESTORATION

TECHNICAL PRODUCT BULLETIN B-55
USEPA, OEM REGULATIONS IMPLEMENTATION DIVISION
ORIGINAL LISTING DATE: SEPTEMBER 10, 1999
"LAND AND SEA RESTORATION PRODUCT 001 (VELITE)"
LAST COMMUNICATION WITH MANUFACTURER: 04/02/2007
LAST ATTEMPT: 8/11/2021

I. NAME, BRAND, OR TRADEMARK
LAND AND SEA RESTORATION PRODUCT 001 (VELITE) Type of Product: Bioremediation Agent (Nutrient Additive)

II. NAME, ADDRESS, AND TELEPHONE NUMBER OF MANUFACTURER/CONTACT
Land and Sea Restoration LLC ADDRESS: updated information required PHONE: updated information required (Mr. T. Shawn Parker)

III. NAME, ADDRESS, AND TELEPHONE NUMBER OF PRIMARY DISTRIBUTORS
Land and Sea Restoration LLC ADDRESS: updated information required PHONE: updated information required (Mr. T. Shawn Parker)

IV. SPECIAL HANDLING AND WORKER PRECAUTIONS FOR STORAGE AND FIELD APPLICATION
1. Flammability: Flash Point 250ºC (482ºF) 2. Ventilation: Normal ventilation 3. Skin and eye contact; protective clothing; treatment in case of contact: In case of eye contact, flush eyes with water or eyewash and refrain from rubbing. For skin contact, wash with mild soap and apply cream if itching or redness occurs. Avoid inhalation. In case of inhalation, seek fresh air. Wear protective clothing and eye goggles. Workers should wear single use nuisance dust masks appropriate for fine particulate dust. 4.a. Maximum storage temperature: 65ºC (150ºF) 4.b. Minimum storage temperature: 0ºC (32ºF) 4.c. Optimum storage temperature range: 0 to 65ºC (32 to 150ºF) 4.d. Temperatures of phase separations and chemical changes: NA

IV. SPECIAL HANDLING AND WORKER PRECAUTIONS FOR STORAGE AND FIELD APPLICATION

1. Flammability:
Flash Point 250ºC (482ºF)
2. Ventilation:
Normal ventilation
3. Skin and eye contact; protective clothing; treatment in case of contact:
In case of eye contact, flush eyes with water or eyewash and refrain from rubbing. For skin contact, wash with mild soap and apply cream if itching or redness occurs. Avoid inhalation. In case of inhalation, seek fresh air. Wear protective clothing and eye goggles. Workers should wear single use nuisance dust masks appropriate for fine particulate dust.
4.a. Maximum storage temperature: 65ºC (150ºF)
4.b. Minimum storage temperature: 0ºC (32ºF)
4.c. Optimum storage temperature range: 0 to 65ºC (32 to 150ºF)
4.d. Temperatures of phase separations and chemical changes: NA

V. SHELF LIFE
More than 5 years in unopened original shipping container, store in a cool, dry place. Avoid moisture prior to use.

VI. RECOMMENDED APPLICATION PROCEDURE
1. Application Method: On water, spread over contaminated area at 1 to 3 ratios. On soil, blend to depth equivalent to contamination level. On hard surfaces, spread over contaminated area. Make sure the spill is completely absorbed. For best results agitate with broom. (Will not damage cured asphalt if used as an absorbent for spills.) Remove and dispose of in accordance with all state and federal laws, after absorption is complete. 2. Concentration/Application Rate: 1 part 001 to 3 parts hydrocarbon. 3. Conditions for Use: NA

VI. RECOMMENDED APPLICATION PROCEDURE

1. Application Method: On water, spread over contaminated area at 1 to 3 ratios. On soil, blend to depth equivalent to contamination level. On hard surfaces, spread over contaminated area. Make sure the spill is completely absorbed. For best results agitate with broom. (Will not damage cured asphalt if used as an absorbent for spills.) Remove and dispose of in accordance with all state and federal laws, after absorption is complete.
2. Concentration/Application Rate: 1 part 001 to 3 parts hydrocarbon.
3. Conditions for Use: NA

VII. TOXICITY AND EFFECTIVENESS

a. Toxicity:
NA

b. Effectiveness:
Bioremediation Agent Effectiveness Test (40 CFR 300.900), Federal Register September 15,1994:

Summary Data Table:

DAYS	PRODUCT 3 Replct/Prod.	TOTAL MEAN Alkane (ppm)	RED.% 28 Days	TOTAL MEAN Aromatics (ppm)	RED.% 28 Days
0	CONTROL 001	13.8239 13.5856	0 0	0.0443 0.0509	0 0
7	CONTROL 001	14.3292 10.1005	0 29.52	0.0473 0.0437	0 0
28	CONTROL 001	14.4675 8.2629	0 42.92	0.0401 0.0273	0 31.92

Results of Gravimetric Analysis:
Percentage (%) Decrease in Weight of Oil on Day 28
Control: 0%
Product (001): 25.18%

VIII. MICROBIOLOGICAL ANALYSIS
1. Listing of all microorganisms by species and percentages in the composition: CONFIDENTIAL 2. Optimum pH, temperature, and salinity ranges for use of the additive: pH: 6 to 8 Temperature: 25 to 30ºC (77ºF to 86ºF) Salinity: Fresh to salt water 3. Minimum and maximum pH, temperature, and salinity levels below or above which the effectiveness of the additive is reduced to half its optimum capacity: pH: NA Temperature: NA Salinity: NA 4. Special nutrient requirements: The product "001" is a nutrient, therefore no nutrients are required. 5. Test results regarding the determination of the presence of the following: Salmonella: Negative Fecal coliform: < 3 Shigella: Negative Staphylococcus Coagulase positive: < 10 Beta hemolytic Streptococci: 1,100

VIII. MICROBIOLOGICAL ANALYSIS

1. Listing of all microorganisms by species and percentages in the composition:
CONFIDENTIAL
2. Optimum pH, temperature, and salinity ranges for use of the additive:
pH: 6 to 8
Temperature: 25 to 30ºC (77ºF to 86ºF)
Salinity: Fresh to salt water
3. Minimum and maximum pH, temperature, and salinity levels below or above which the effectiveness of the additive is reduced to half its optimum capacity:
pH: NA
Temperature: NA
Salinity: NA
4. Special nutrient requirements:
The product "001" is a nutrient, therefore no nutrients are required.
5. Test results regarding the determination of the presence of the following:
Salmonella: Negative
Fecal coliform: < 3
Shigella: Negative
Staphylococcus Coagulase positive: < 10
Beta hemolytic Streptococci: 1,100

IX. PHYSICAL PROPERTIES
1. Flash Point: 250ºC (482ºF) 2. Pour Point: NA 3. Viscosity: NA 4. Specific Gravity: NA 5. pH: NA 6. Surface Active Agents: NA 7. Solvents: NA 8. Additives: NA 9. Solubility in Water: NA

X. ANALYSIS FOR HEAVY METALS, CYANIDE, AND CHLORINATED HYDROCARBONS
NA

LAND AND SEA RESTORATION

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